Competence for Transfection in Staphylococcus aureus

Transfection of Staphylococcus aureus with bacteriophage deoxyribonucleic acid.

Staphylococcus aureus cells of strain 8325 (N) are competent for phage deoxyribonucleic acid (DNA) when harvested in the early exponential growth phase. Phenotypic expression of the competence requires divalent cations, and calcium ions are most effective. Treatment of phage DNA with deoxyribonuclease completely destroys infectivity and heat-denaturated DNA is not infectious. The highest freque...

Transfection of lysostaphin-treated cells of Staphylococcus aureus.

After treatment with 1 unit of lysostaphin per ml for 3 min, two strains of Staphylococcus aureus, 233 and PS 44A HJD, were transfected with phenol-extracted deoxyribonucleic acid (DNA) from the staphylococcal bacteriophages, 53 and 44A HJD, respectively. The number of transfected cells was low in both systems, approximately two in 10(7) enzyme-treated cells. There was a saturation effect at hi...

Staphylococcus Aureus Carriage in Patients with Psoriasis

Background: The aggravating role of Staphylococcus aureus is well known in atopic dermatitis but has not yet been proven in psoriasis. The role of Staphylococcus aureus superantigens is emphasized in the initiation, maintenance and complications of psoriasis. We investigated the frequency of nasal, axillary, and perineal carriage of Staphylococcus aureus (SA) in patients with psoriasis and its ...

Vancomycin Resistance in Staphylococcus aureus Strains

Formation in Staphylococcus aureus

15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 *For correspondence: Andrew McBain, School of Pharmacy, The University of Manchester, 30 Oxford Road, Manchester M13 9PT, UK. Tel: 00 44 161 275 2360; Fax: 00 44 161 275 2396; 31 Email: [email protected] 32 33 Copyright © 2012, American Society for Microbiology. All Rights Reserved. Antimicrob. Agents Chemother. doi:10.1128/AAC.05904-11 ...